Hotspot

rps15-ycf1

SSC · 133008-133548 · 541 bp

Back to Bruguiera

Pi 0.0157

MarkerSeek score 53.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 541
variable_sites: 20
pi: 0.0157
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGACTCCCGAATATCCAGCT	AAAACTGCAAGCCCGTTTTG	50.0	45.0	57.9	58.3	985-999 bp	1.0	81.5
rps15-ycf1_p2	GATGAGTCGAAGAACGCCAT	AAAACTGCAAGCCCGTTTTG	50.0	45.0	58.1	58.3	1205-1210 bp	1.0	81.1
rps15-ycf1_p3	CATAGGGGTTTCCTCGCTTC	AAAACTGCAAGCCCGTTTTG	55.0	45.0	58.0	58.3	1125-1126 bp	0.833333	72.2
rps15-ycf1_p4	CATAGGGGTTTCCTCGCTTC	ACTGCAAGCCCGTTTTGTAT	55.0	45.0	58.0	58.4	1122-1123 bp	0.833333	71.9
rps15-ycf1_p5	CATAGGGGTTTCCTCGCTTC	AAAAACTGCAAGCCCGTTTT	55.0	40.0	58.0	57.6	1126-1127 bp	0.833333	71.8