Hotspot

rpl32-trnL-UAG

SSC · 121571-123507 · 1937 bp

Back to Bruguiera

Pi 0.042

MarkerSeek score 75.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1937
variable_sites: 183
pi: 0.042
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAAATCAAACGGTGGAATAA	AGCGTGTCTACCGATTTCAC	28.6	50.0	52.2	58.0	1620-2374 bp	1.0	51.0
rpl32-trnL-UAG_p2	AAAAATCAAACGGTGGAATAA	CAGCGTGTCTACCGATTTCA	28.6	50.0	52.2	58.0	1621-2375 bp	1.0	51.0
rpl32-trnL-UAG_p3	AAAAATCAAACGGTGGAATAA	TCCTAAGAGCAGCGTGTCTA	28.6	50.0	52.2	57.9	1630-2384 bp	1.0	50.9
rpl32-trnL-UAG_p4	AAAAATCAAACGGTGGAATAA	CTAAGAGCAGCGTGTCTACC	28.6	55.0	52.2	57.8	1628-2382 bp	1.0	50.9
rpl32-trnL-UAG_p5	AAAAATCAAACGGTGGAATAA	AAAGATGTTATGCCGCCACT	28.6	45.0	52.2	57.9	1681-2435 bp	1.0	50.8