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psbZ-trnG-GCC

LSC · 40262-41432 · 1171 bp

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Pi 0.0204

MarkerSeek score 70.60

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1171
variable_sites: 49
pi: 0.0204
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	972-1446 bp	1.0	85.6
psbZ-trnG-GCC_p2	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	968-1442 bp	1.0	83.9
psbZ-trnG-GCC_p3	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	971-1445 bp	1.0	82.8
psbZ-trnG-GCC_p4	TCTCCTGATGGTTGGTCGAG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.8	57.8	970-1444 bp	1.0	79.2
psbZ-trnG-GCC_p5	CTTCTCCTGATGGTTGGTCG	CGGATAGCGGGAATCGAAC	55.0	57.9	58.0	58.2	994-1468 bp	1.0	77.3