Hotspot

trnE-UUC-trnT-GGU

LSC · 33540-34871 · 1332 bp

Back to Bruguiera

Pi 0.0117

MarkerSeek score 52.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1332
variable_sites: 32
pi: 0.0117
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	1201-1437 bp	1.0	83.8
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	1180-1416 bp	1.0	83.7
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	1198-1434 bp	1.0	82.7
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.7	58.2	1177-1413 bp	1.0	82.6
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TTGAACCGATGACTTACGCC	55.0	50.0	58.3	58.3	1203-1439 bp	1.0	81.6