Hotspot

trnK-UUU-rps16

LSC · 4354-5038 · 685 bp

Back to Brugmansia

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
685
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.2 57.5 817 bp 1.0 74.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.2 57.5 816 bp 1.0 74.9
trnK-UUU-rps16_p3 TCAGTCGTGGTCTTCCAAAC TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.8 57.5 902 bp 1.0 74.4
trnK-UUU-rps16_p4 CAGTCGTGGTCTTCCAAACT TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.8 57.5 901 bp 1.0 74.3
trnK-UUU-rps16_p5 AAAAGACACTAGCCGCACTT TTGAAAAAGGGGCTCAACCT 45.0 45.0 57.7 57.5 838 bp 1.0 74.2