Hotspot

matK-trnK-UUU

LSC · 3610-4316 · 707 bp

Back to Brugmansia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGTTGTGTTGTTGCGAGCTA	AACGGTAGAGTACTCGGCTT	45.0	50.0	58.1	58.2	783 bp	1.0	78.2
matK-trnK-UUU_p2	AGTTGTGTTGTTGCGAGCTA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	58.1	58.2	782 bp	1.0	78.2
matK-trnK-UUU_p3	GGAAGTTGTGTTGTTGCGAG	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.9	58.2	786 bp	1.0	77.8
matK-trnK-UUU_p4	GGAAGTTGTGTTGTTGCGAG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	785 bp	1.0	77.8
matK-trnK-UUU_p5	GCGAGCTATCTGGCTGTAAA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.8	58.2	770 bp	1.0	76.9