Hotspot

rps14

LSC · 38913-39215 · 303 bp

Back to Brownlowia

Pi 0

MarkerSeek score 23.70

Primer available yes

Label rps14

Feature Metadata

label_name: rps14
feature_type: gene
region: LSC
length_bp: 303
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14_p1	GCGCCCCTCTTCCATATATG	ACATCTAGGATCCGACTTGT	55.0	45.0	58.0	55.4	471 bp	1.0	58.6
rps14_p2	ATGCGCCCCTCTTCCATATA	ACATCTAGGATCCGACTTGT	50.0	45.0	58.3	55.4	473 bp	1.0	56.1
rps14_p3	TGCGCCCCTCTTCCATATAT	ACATCTAGGATCCGACTTGT	50.0	45.0	58.3	55.4	472 bp	1.0	56.1
rps14_p4	GCGCCCCTCTTCCATATATG	TCTACATCTAGGATCCGACTTGT	55.0	43.5	58.0	57.9	474 bp	1.0	55.4
rps14_p5	GGCCCCTCTATGATCATCGA	ACATCTAGGATCCGACTTGT	55.0	45.0	58.4	55.4	413 bp	1.0	55.4