Hotspot

rpoC2

LSC · 17509-21582 · 4074 bp

Back to Brownlowia

Pi 0.000245

MarkerSeek score 54.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4074
variable_sites: 1
pi: 0.000245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CGCGAATCTCAGCAATCACT	TTACAGGGGGTACTGCAGAA	50.0	50.0	58.7	58.0	243 bp	1.0	74.3
rpoC2_p2	CGCGAATCTCAGCAATCACT	TTTACAGGGGGTACTGCAGA	50.0	50.0	58.7	58.0	244 bp	1.0	74.3
rpoC2_p3	CGCGAATCTCAGCAATCACT	GAACATGTACGAGCCCCTTT	50.0	50.0	58.7	57.9	226 bp	1.0	73.8
rpoC2_p4	CGCGAATCTCAGCAATCACT	CATTGGAGAACCGGGAACTC	50.0	55.0	58.7	58.3	299 bp	1.0	72.5
rpoC2_p5	CGCGAATCTCAGCAATCACT	AACTTTTCATACCGGCGGAG	50.0	50.0	58.7	58.3	266 bp	1.0	72.5