Hotspot

clpP1

LSC · 73858-75987 · 2130 bp

Back to Brownlowia

Pi 0

MarkerSeek score 57.50

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2130
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TGGATTGGTGTTAAATCGTTGC	ACGCTTCCACATCAAAGTGA	40.9	45.0	57.8	57.7	2215-2216 bp	1.0	60.7
clpP1_p2	GGATTGGTGTTAAATCGTTGCT	ACGCTTCCACATCAAAGTGA	40.9	45.0	57.6	57.7	2214-2215 bp	1.0	58.5
clpP1_p3	GGATTGGTGTTAAATCGTTGC	ACGCTTCCACATCAAAGTGA	42.9	45.0	56.1	57.7	2214-2215 bp	1.0	55.0
clpP1_p4	GGTGTTAAATCGTTGCTATTGCT	ACGCTTCCACATCAAAGTGA	39.1	45.0	58.0	57.7	2209-2210 bp	1.0	53.9
clpP1_p5	TGTGGATTGGTGTTAAATCGTTG	ACGCTTCCACATCAAAGTGA	39.1	45.0	57.9	57.7	2217-2218 bp	1.0	53.1