Hotspot

clpP1-psbB

LSC · 75988-76396 · 409 bp

Back to Brownlowia

Pi 0

MarkerSeek score 25.00

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 409
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	505 bp	1.0	78.1
clpP1-psbB_p2	CCCCGGATTTCGAAAAGGAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.2	58.2	471 bp	1.0	76.7
clpP1-psbB_p3	ACCCAAACTGCATCTTCCTC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	491 bp	1.0	76.4
clpP1-psbB_p4	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	508 bp	1.0	76.2
clpP1-psbB_p5	TACGTCAACCCAAACTGCAT	CGATACCAAGGCAAACCCAT	45.0	50.0	57.7	58.2	498 bp	1.0	76.0