Hotspot

trnW-CCA

LSC · 68613-68686 · 74 bp

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Pi 0.0135

MarkerSeek score 60.30

Primer available yes

Label trnW-CCA

Feature Metadata

label_name: trnW-CCA
feature_type: tRNA
region: LSC
length_bp: 74
variable_sites: 1
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA_p1	GAATCCACAGGAGGTCCAAA	CCAGATGTATTGGTGGGGTC	50.0	55.0	57.1	57.7	178 bp	1.0	70.6
trnW-CCA_p2	GAATCCACAGGAGGTCCAAA	ATGCCAGATGTATTGGTGGG	50.0	50.0	57.1	57.6	181 bp	1.0	70.4
trnW-CCA_p3	GAATCCACAGGAGGTCCAAA	TGGTGGGGTCACAAAAATGA	50.0	45.0	57.1	57.5	168 bp	1.0	69.8
trnW-CCA_p4	ATTCGAATCCACAGGAGGTC	CCAGATGTATTGGTGGGGTC	50.0	55.0	57.0	57.7	182 bp	1.0	69.7
trnW-CCA_p5	ATTCGAATCCACAGGAGGTC	ATGCCAGATGTATTGGTGGG	50.0	50.0	57.0	57.6	185 bp	1.0	69.6