Hotspot

trnD-GUC

LSC · 32505-32578 · 74 bp

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Pi 0.0135

MarkerSeek score 60.30

Primer available yes

Label trnD-GUC

Feature Metadata

label_name: trnD-GUC
feature_type: tRNA
region: LSC
length_bp: 74
variable_sites: 1
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC_p1	AGAAAGGGGGTCCTAGTTAGG	TCAATAGATCAGTCCCGCCT	52.4	50.0	57.9	57.9	277 bp	1.0	70.5
trnD-GUC_p2	AGAAAGGGGGTCCTAGTTAGG	CGCCTCTGTCAGTTACAACA	52.4	50.0	57.9	57.9	262 bp	1.0	70.1
trnD-GUC_p3	AGAAAGGGGGTCCTAGTTAGG	ATAGATCAGTCCCGCCTCTG	52.4	55.0	57.9	58.4	274 bp	1.0	68.2
trnD-GUC_p4	AGAAAGGGGGTCCTAGTTAGG	AATAGATCAGTCCCGCCTCT	52.4	50.0	57.9	57.6	275 bp	1.0	68.1
trnD-GUC_p5	AGAAAGGGGGTCCTAGTTAGG	CAATAGATCAGTCCCGCCTC	52.4	55.0	57.9	57.6	276 bp	1.0	67.7