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rpoC2

LSC · 17625-21653 · 4029 bp

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Pi 0.000248

MarkerSeek score 54.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4029
variable_sites: 1
pi: 0.000248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AGAAATCCCTCGAATGGTGC	GGGAGCTAGAGGAAATGCAT	50.0	50.0	58.0	57.1	280 bp	1.0	72.4
rpoC2_p2	GCCGCAATCTGTTCTACGTA	GGGAGCTAGAGGAAATGCAT	50.0	50.0	58.1	57.1	262 bp	1.0	72.1
rpoC2_p3	AGAAATCCCTCGAATGGTGC	GTCTTTTTCGGGAGCTAGAGG	50.0	52.4	58.0	58.1	289 bp	1.0	71.1
rpoC2_p4	GCCGCAATCTGTTCTACGTA	GTCTTTTTCGGGAGCTAGAGG	50.0	52.4	58.1	58.1	271 bp	1.0	70.8
rpoC2_p5	AGAAATCCCTCGAATGGTGC	TCTTTTTCGGGAGCTAGAGGA	50.0	47.6	58.0	58.2	288 bp	1.0	70.5