Hotspot

petL-petG

LSC · 68210-68375 · 166 bp

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Pi 0

MarkerSeek score 33.80

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TCTTGCCTCTTTTCATGCCT	GTTCCAACTGATCACCACGT	45.0	50.0	57.4	58.1	385 bp	1.0	74.3
petL-petG_p2	TCTTGCCTCTTTTCATGCCT	TTCCAACTGATCACCACGTC	45.0	50.0	57.4	57.8	384 bp	1.0	74.0
petL-petG_p3	TCTTGCCTCTTTTCATGCCT	AGTTCCAACTGATCACCACG	45.0	50.0	57.4	57.8	386 bp	1.0	73.9
petL-petG_p4	ATCTTGCCTCTTTTCATGCCT	GTTCCAACTGATCACCACGT	42.9	50.0	57.9	58.1	386 bp	1.0	70.3
petL-petG_p5	CGGCTCTATTTATTGGGCTGA	GTTCCAACTGATCACCACGT	47.6	50.0	57.9	58.1	316 bp	1.0	70.0