Hotspot

trnS-GCU-trnG-UCC

LSC · 8261-9171 · 911 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
911
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ATGAATCAAACGAGGGACCC 50.0 50.0 58.0 57.6 1024 bp 1.0 76.6
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ATGAATCAAACGAGGGACCC 50.0 50.0 58.0 57.6 1023 bp 1.0 76.6
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA ATGAATCAAACGAGGGACCC 50.0 50.0 58.2 57.6 1078 bp 1.0 75.4
trnS-GCU-trnG-UCC_p4 AGCAATCCGACGCTTTAGTC ATGAATCAAACGAGGGACCC 50.0 50.0 58.4 57.6 1026 bp 1.0 73.8
trnS-GCU-trnG-UCC_p5 CGACGCTTTAGTCCACTCAG ATGAATCAAACGAGGGACCC 55.0 50.0 58.4 57.6 1019 bp 1.0 73.6