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matK-trnK-UUU

LSC · 3619-4348 · 730 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
730
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TTCCCGAAAGATAAGTGGGT TCGGGTTGCTAACTCAATGG 45.0 50.0 56.1 57.9 847 bp 1.0 64.3
matK-trnK-UUU_p2 TTCCCGAAAGATAAGTGGGT CGGGTTGCTAACTCAATGGT 45.0 50.0 56.1 58.2 846 bp 1.0 63.7
matK-trnK-UUU_p3 AGGAGGTCATATTGCTGAAACC TCGGGTTGCTAACTCAATGG 45.5 50.0 58.1 57.9 825 bp 1.0 62.3
matK-trnK-UUU_p4 GGAGGTCATATTGCTGAAACCT TCGGGTTGCTAACTCAATGG 45.5 50.0 58.1 57.9 824 bp 1.0 62.3
matK-trnK-UUU_p5 GGAGGTCATATTGCTGAAACCT CGGGTTGCTAACTCAATGGT 45.5 50.0 58.1 58.2 823 bp 1.0 61.7