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trnH-GUG-psbA

LSC · 84-440 · 357 bp

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Pi 0
MarkerSeek score 34.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
357
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCCAT 55.0 55.0 58.1 58.0 423 bp 1.0 78.9
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCCAT 50.0 55.0 57.9 58.0 437 bp 1.0 78.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCCAT 50.0 55.0 57.9 58.0 436 bp 1.0 78.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCCAT 50.0 55.0 57.9 58.0 438 bp 1.0 78.9
trnH-GUG-psbA_p5 TATGTTATGGGCGAACGACG CCTCTAGACCTAGCTGCCAT 50.0 55.0 58.2 58.0 485 bp 1.0 78.7