Hotspot

ycf1

SSC · 130158-135812 · 5655 bp

Back to Broussonetia

Pi 0.0166

MarkerSeek score 42.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5655
variable_sites: 232
pi: 0.0166
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTCCGTTTCCATGTTGGTC	AAATTATCGCAAACCCCGGT	50.0	45.0	58.1	58.2	1204-1219 bp	1.0	81.6
ycf1_p2	ACTCCGTTTCCATGTTGGTC	AATTATCGCAAACCCCGGTT	50.0	45.0	58.1	58.2	1203-1218 bp	1.0	81.6
ycf1_p3	TCGAGATTCGATACGGGACA	AAATTATCGCAAACCCCGGT	50.0	45.0	58.0	58.2	860 bp	0.857143	74.8
ycf1_p4	TCGAGATTCGATACGGGACA	AATTATCGCAAACCCCGGTT	50.0	45.0	58.0	58.2	859 bp	0.857143	74.8
ycf1_p5	TCGAGATTCGATACGGGACA	CAAATTATCGCAAACCCCGG	50.0	50.0	58.0	57.8	861 bp	0.857143	74.5