Hotspot

trnL-UAG-ccsA

SSC · 120393-120495 · 103 bp

Back to Broussonetia

Pi 0.0601

MarkerSeek score 60.90

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 103
variable_sites: 16
pi: 0.0601
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGCTCTAAAGTTGAAAATATCAT	50.0	26.1	57.9	52.2	186-194 bp	1.0	47.3
trnL-UAG-ccsA_p2	GTGAAATCGGTAGACACGCT	ATGCTCTAAAGTTGAAAATATCA	50.0	26.1	58.0	52.2	197-205 bp	1.0	46.9
trnL-UAG-ccsA_p3	GTGAAATCGGTAGACACGCT	TGCTCTAAAGTTGAAAATATCAT	50.0	26.1	58.0	52.2	196-204 bp	1.0	46.9
trnL-UAG-ccsA_p4	TGAAATCGGTAGACACGCTG	ATGCTCTAAAGTTGAAAATATCA	50.0	26.1	58.0	52.2	196-204 bp	1.0	46.9
trnL-UAG-ccsA_p5	TGAAATCGGTAGACACGCTG	TGCTCTAAAGTTGAAAATATCAT	50.0	26.1	58.0	52.2	195-203 bp	1.0	46.9