Hotspot

ndhF-rpl32

SSC · 117053-118389 · 1337 bp

Back to Broussonetia

Pi 0.0357

MarkerSeek score 68.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1337
variable_sites: 111
pi: 0.0357
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCGAAGATCTTTTGTTGCC	CTCTTGCTATTGGTCCGGTC	50.0	55.0	58.0	58.1	2002-2094 bp	0.857143	79.5
ndhF-rpl32_p2	GGCGAAGATCTTTTGTTGCC	CTTGCTATTGGTCCGGTCAA	50.0	50.0	58.0	57.9	2000-2092 bp	0.857143	79.1
ndhF-rpl32_p3	TAGGGCGTGTGAACCAAAAA	CTCTTGCTATTGGTCCGGTC	45.0	55.0	57.9	58.1	2398-2490 bp	0.857143	78.6
ndhF-rpl32_p4	GAAGTAAGCGGGTCAACCAA	CTCTTGCTATTGGTCCGGTC	50.0	55.0	58.1	58.1	2171-2263 bp	0.857143	78.4
ndhF-rpl32_p5	TAGGGCGTGTGAACCAAAAA	CTTGCTATTGGTCCGGTCAA	45.0	50.0	57.9	57.9	2396-2488 bp	0.857143	78.2