Hotspot

psbZ-trnG-GCC

LSC · 38769-39483 · 715 bp

Back to Broussonetia

Pi 0.0292

MarkerSeek score 62.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 715
variable_sites: 47
pi: 0.0292
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CAAGACCGGAGCTATCAACC	CGACGTTGTTTTTCGACTGG	55.0	50.0	58.1	58.0	1706-1860 bp	1.0	87.1
psbZ-trnG-GCC_p2	CAAGACCGGAGCTATCAACC	CCTATACGCCTTCATCGACG	55.0	55.0	58.1	58.0	1721-1875 bp	1.0	86.8
psbZ-trnG-GCC_p3	GGCCATCTCTCCGAAAGAAG	CGACGTTGTTTTTCGACTGG	55.0	50.0	58.1	58.0	1825-1836 bp	0.857143	77.0
psbZ-trnG-GCC_p4	GGCCATCTCTCCGAAAGAAG	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.1	58.0	1495-1504 bp	0.857143	76.8
psbZ-trnG-GCC_p5	GGCCATCTCTCCGAAAGAAG	CCTATACGCCTTCATCGACG	55.0	55.0	58.1	58.0	1840-1851 bp	0.857143	76.7