Hotspot

trnK-UUU-rps16

LSC · 4429-5547 · 1119 bp

Back to Broussonetia

Pi 0.0233

MarkerSeek score 60.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1119
variable_sites: 63
pi: 0.0233
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	TTGAAAAAGGTGCTCAGCCT	50.0	45.0	57.9	57.9	1317-1331 bp	1.0	85.5
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	TTGAAAAAGGTGCTCAGCCT	50.0	45.0	57.9	57.9	1316-1330 bp	1.0	85.5
trnK-UUU-rps16_p3	GTCGCGGTCTTACAAACTCT	GCCGTACGAGGAGAAAACTT	50.0	50.0	57.9	57.9	1508-1522 bp	1.0	84.7
trnK-UUU-rps16_p4	AGTCGCGGTCTTACAAACTC	TTTTCTTGAGCCGTACGAGG	50.0	50.0	57.9	57.9	1518-1532 bp	1.0	84.6
trnK-UUU-rps16_p5	GTCGCGGTCTTACAAACTCT	TTTTCTTGAGCCGTACGAGG	50.0	50.0	57.9	57.9	1517-1531 bp	1.0	84.6