Hotspot

ndhH

IRb · 102664-102999 · 336 bp

Pi 0.0779

MarkerSeek score 73.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: IRb
length_bp: 336
variable_sites: 45
pi: 0.0779
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TTTGATGAGCCTCGCTTTGA	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	57.8	58.0	443-553 bp	1.0	89.9
ndhH_p2	CTTTTGGGAAAACGTCGACG	ATGGAGTGGGTCTTGCTAGT	50.0	50.0	58.0	58.0	631-742 bp	1.0	89.5
ndhH_p3	TCGACGTTTGCTGGCTTATT	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	58.2	58.0	617-728 bp	1.0	88.2
ndhH_p4	CTTTTGGGAAAACGTCGACG	TGGAGTGGGTCTTGCTAGTT	50.0	50.0	58.0	58.3	630-741 bp	1.0	87.7
ndhH_p5	GCCGGAAGATGGATGCATAT	ATGGAGTGGGTCTTGCTAGT	50.0	50.0	57.9	58.0	1114-1225 bp	1.0	86.6