Hotspot

rbcL-psaI

LSC · 56357-58069 · 1713 bp

Back to Bromus

Pi 0.0162
MarkerSeek score 58.20
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
1713
variable_sites
54
pi
0.0162
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 GCCGGTAGATACTATCGATT GTCATTGCAATTGCCGGAAA 45.0 45.0 54.0 57.9 869-1806 bp 0.8 45.5
rbcL-psaI_p2 GCCGGTAGATACTATCGATT GAAGTCATTGCAATTGCCGG 45.0 50.0 54.0 58.4 872-1809 bp 0.8 43.2
rbcL-psaI_p3 GCCGGTAGATACTATCGATT AGAAGTCATTGCAATTGCCG 45.0 45.0 54.0 57.4 873-1810 bp 0.8 41.0
rbcL-psaI_p4 GCCGGTAGATACTATCGATT AAGTCATTGCAATTGCCGGA 45.0 45.0 54.0 58.7 871-1808 bp 0.8 40.2
rbcL-psaI_p5 GCCGGTAGATACTATCGATT AGTCATTGCAATTGCCGGAA 45.0 45.0 54.0 58.7 870-1807 bp 0.8 40.2