Hotspot

ndhC-trnV-UAC

LSC · 50259-51177 · 919 bp

Back to Bromus

Pi 0.0201
MarkerSeek score 65.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
919
variable_sites
37
pi
0.0201
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 36.4 55.0 56.1 57.9 964-1074 bp 1.0 53.0
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA CGGTTCGAATCCGTATAGCC 36.4 55.0 56.1 58.3 965-1075 bp 1.0 51.2
ndhC-trnV-UAC_p3 CATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 38.1 55.0 54.6 57.9 963-1073 bp 1.0 49.0
ndhC-trnV-UAC_p4 ATCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 965-1075 bp 1.0 49.0
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 964-1074 bp 1.0 49.0