Hotspot

trnC-GCA-rpoB

LSC · 18669-19833 · 1165 bp

Back to Bromus

Pi 0.0159
MarkerSeek score 64.50
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1165
variable_sites
35
pi
0.0159
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG ATTCCCTCGTTTCCATTCCG 50.0 50.0 58.1 58.0 1222-1235 bp 1.0 82.7
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG TGTGGACATTCCCTCGTTTC 50.0 50.0 58.1 57.8 1229-1242 bp 1.0 81.6
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1237-1250 bp 1.0 80.4
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1236-1249 bp 1.0 80.4
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG AAGTTCTTCTGCCAAGGCTT 50.0 45.0 58.1 57.6 1301-1314 bp 1.0 79.8