Hotspot

petN-trnC-GCA

LSC · 17667-18597 · 931 bp

Back to Bromus

Pi 0.0158
MarkerSeek score 67.30
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
931
variable_sites
27
pi
0.0158
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGTCCACTCCTTCCCCATAC AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 913-1044 bp 1.0 76.8
petN-trnC-GCA_p2 GTCCACTCCTTCCCCATACT AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 912-1043 bp 1.0 76.8
petN-trnC-GCA_p3 AGCCCAAGCGAGACTTACTA AGTTCAAATCTGGGTGCCG 50.0 52.6 58.2 58.1 854-985 bp 1.0 76.7
petN-trnC-GCA_p4 GTCCACTCCTTCCCCATACT CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 914-1045 bp 1.0 76.4
petN-trnC-GCA_p5 AGTCCACTCCTTCCCCATAC CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 915-1046 bp 1.0 76.3