Hotspot

trnG-UCC-trnT-GGU

LSC · 13520-14739 · 1220 bp

Back to Bromus

Pi 0.0126
MarkerSeek score 63.50
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1220
variable_sites
28
pi
0.0126
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT ACCACTGAGTTAAAAGGGCT 38.1 45.0 55.5 56.7 1217-1300 bp 1.0 46.7
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1254-1337 bp 1.0 46.5
trnG-UCC-trnT-GGU_p3 GAACGAATCACACTTTTACCACT ACCACTGAGTTAAAAGGGCT 39.1 45.0 57.3 56.7 1219-1302 bp 1.0 45.8
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1250-1333 bp 1.0 45.7
trnG-UCC-trnT-GGU_p5 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1251-1334 bp 1.0 45.7