Hotspot

trnS-GCU-psbD

LSC · 7647-8557 · 911 bp

Back to Bromus

Pi 0.0114
MarkerSeek score 66.10
Primer available yes
Label trnS-GCU-psbD

Feature Metadata

label_name
trnS-GCU-psbD
feature_type
igs
region
LSC
length_bp
911
variable_sites
19
pi
0.0114
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-psbD_p1 ATTAGCAATCCGCCGCTTTA GAGCAATAGGCCAGACCATC 45.0 55.0 58.0 58.1 1051-1077 bp 1.0 82.2
trnS-GCU-psbD_p2 ATTAGCAATCCGCCGCTTTA ACAAAAACGAAACGGTCCCT 45.0 45.0 58.0 58.2 1029-1055 bp 1.0 81.2
trnS-GCU-psbD_p3 ATTAGCAATCCGCCGCTTTA CCCTTCGTAACCAGTCATCC 45.0 55.0 58.0 57.7 1013-1039 bp 1.0 80.7
trnS-GCU-psbD_p4 GAGCGGAAAGAGAGGGATTC GAGCAATAGGCCAGACCATC 55.0 55.0 57.8 58.1 1102-1128 bp 1.0 80.5
trnS-GCU-psbD_p5 ATTAGCAATCCGCCGCTTTA AGGCCAGACCATCCTACAAA 45.0 50.0 58.0 58.3 1044-1070 bp 1.0 80.5