Hotspot

trnK-UUU-rps16

LSC · 3912-4492 · 581 bp

Back to Bromus

Pi 0.012
MarkerSeek score 64.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
581
variable_sites
13
pi
0.012
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG GCTCAACCGACAAGAACTGT 50.0 50.0 58.6 58.4 686-705 bp 1.0 76.6
trnK-UUU-rps16_p2 AGTCGCACTTAAAAGCCGAG GTGCTCAACCGACAAGAACT 50.0 50.0 58.6 58.4 688-707 bp 1.0 76.6
trnK-UUU-rps16_p3 AGTCGCACTTAAAAGCCGAG TGCTCAACCGACAAGAACTG 50.0 50.0 58.6 58.4 687-706 bp 1.0 76.6
trnK-UUU-rps16_p4 TCGCACTTAAAAGCCGAGTA GCTCAACCGACAAGAACTGT 45.0 50.0 57.3 58.4 684-703 bp 1.0 75.4
trnK-UUU-rps16_p5 TCGCACTTAAAAGCCGAGTA GTGCTCAACCGACAAGAACT 45.0 50.0 57.3 58.4 686-705 bp 1.0 75.4