Hotspot

rpl22

LSC · 87453-87872 · 420 bp

Back to Brocchinia

Pi 0.0165

MarkerSeek score 46.80

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 420
variable_sites: 16
pi: 0.0165
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TATGCGTGCAATTCCTTCGA	TCTGACTTTCGCGAGACATG	45.0	50.0	58.0	58.0	813-820 bp	1.0	85.1
rpl22_p2	CGTGCAATTCCTTCGAAACC	TCTGACTTTCGCGAGACATG	50.0	50.0	58.0	58.0	809-816 bp	1.0	84.9
rpl22_p3	TATGCGTGCAATTCCTTCGA	TAGTAACTTGGTCTCGGGCA	45.0	50.0	58.0	58.1	952-959 bp	1.0	83.9
rpl22_p4	TATGCGTGCAATTCCTTCGA	GTTATCCTGCGCTTGGAAGA	45.0	50.0	58.0	58.0	1376-1384 bp	1.0	83.3
rpl22_p5	CGTGCAATTCCTTCGAAACC	GTTATCCTGCGCTTGGAAGA	50.0	50.0	58.0	58.0	1372-1380 bp	1.0	83.1