Hotspot

ndhF-rpl32

SSC · 116975-118060 · 1086 bp

Back to Brocchinia

Pi 0.0384
MarkerSeek score 74.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1086
variable_sites
92
pi
0.0384
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGAAAAGGTATTATCCACGCA CCGATTTCGCTAAAGAAAAAGC 38.1 40.9 55.0 56.9 1209-1245 bp 0.833333 42.9
ndhF-rpl32_p2 AGAAAAGGTATTATCCACGCA CCGATTTCGCTAAAGAAAAAGCT 38.1 39.1 55.0 58.3 1209-1245 bp 0.833333 42.9
ndhF-rpl32_p3 AGAAAAGGTATTATCCACGCA AGAAAAAGCTTTTACTGCAGC 38.1 38.1 55.0 55.6 1196-1232 bp 0.833333 42.9
ndhF-rpl32_p4 AGAAAAGGTATTATCCACGCAT CCGATTTCGCTAAAGAAAAAGC 36.4 40.9 55.6 56.9 1209-1245 bp 0.833333 42.9
ndhF-rpl32_p5 AAGAAAAGGTATTATCCACGCA CCGATTTCGCTAAAGAAAAAGC 36.4 40.9 55.8 56.9 1210-1246 bp 0.833333 42.8