Hotspot

rpl22-rps19

IRb · 87873-87983 · 111 bp

Back to Brocchinia

Pi 0.0595

MarkerSeek score 71.80

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 111
variable_sites: 17
pi: 0.0595
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TATGCGTGCAATTCCTTCGA	TCTGACTTTCGCGAGACATG	45.0	50.0	58.0	58.0	813-820 bp	1.0	85.1
rpl22-rps19_p2	TATGCGTGCAATTCCTTCGA	GTTATCCTGCGCTTGGAAGA	45.0	50.0	58.0	58.0	1376-1384 bp	1.0	83.3
rpl22-rps19_p3	TATGCGTGCAATTCCTTCGA	GGGTAGAGCCGGATCTAAGT	45.0	55.0	58.0	58.0	1513-1521 bp	1.0	83.1
rpl22-rps19_p4	CGTGCAATTCCTTCGAAACC	GGGTAGAGCCGGATCTAAGT	50.0	55.0	58.0	58.0	1509-1517 bp	1.0	82.9
rpl22-rps19_p5	CTCCTATTGGCTTCGGCTTT	GGGTAGAGCCGGATCTAAGT	50.0	55.0	58.0	58.0	1149-1154 bp	1.0	82.8