Hotspot

rps3-rpl22

LSC · 87402-87452 · 51 bp

Back to Brocchinia

Pi 0.052

MarkerSeek score 70.00

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 51
variable_sites: 4
pi: 0.052
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TATGCGTGCAATTCCTTCGA	TCTGACTTTCGCGAGACATG	45.0	50.0	58.0	58.0	813-820 bp	1.0	85.1
rps3-rpl22_p2	CGTGCAATTCCTTCGAAACC	TCTGACTTTCGCGAGACATG	50.0	50.0	58.0	58.0	809-816 bp	1.0	84.9
rps3-rpl22_p3	TATGCGTGCAATTCCTTCGA	TAGTAACTTGGTCTCGGGCA	45.0	50.0	58.0	58.1	952-959 bp	1.0	83.9
rps3-rpl22_p4	TATGCGTGCAATTCCTTCGA	AAAGCCGAAGCCAATAGGAG	45.0	50.0	58.0	58.0	383-387 bp	1.0	82.8
rps3-rpl22_p5	CGTGCAATTCCTTCGAAACC	AAAGCCGAAGCCAATAGGAG	50.0	50.0	58.0	58.0	379-383 bp	1.0	82.5