Hotspot

rps8-rpl14

LSC · 84396-84585 · 190 bp

Back to Brocchinia

Pi 0.0381

MarkerSeek score 62.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 190
variable_sites: 14
pi: 0.0381
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GTCTTTCGATGTCGCAGAGT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.0	58.0	446-520 bp	1.0	87.1
rps8-rpl14_p2	TGTCGCAGAGTTGAAACCAA	GAACTCGAGTTTTTGGTGCG	45.0	50.0	58.0	58.0	437-511 bp	1.0	87.0
rps8-rpl14_p3	GTCTTTCGATGTCGCAGAGT	CCTGTTACAGTAAGACCCGC	50.0	55.0	58.0	58.0	1055-1128 bp	1.0	85.2
rps8-rpl14_p4	TGTCGCAGAGTTGAAACCAA	CCTGTTACAGTAAGACCCGC	45.0	55.0	58.0	58.0	1046-1119 bp	1.0	85.1
rps8-rpl14_p5	TAAATTCGTAAGCCGGGTCG	CCTGTTACAGTAAGACCCGC	50.0	55.0	58.1	58.0	1121-1194 bp	1.0	84.6