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psbE-petL

LSC · 67833-69408 · 1576 bp

Back to Brocchinia

Pi 0.0203

MarkerSeek score 63.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1576
variable_sites: 66
pi: 0.0203
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	872-1772 bp	1.0	83.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	870-1770 bp	1.0	83.1
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.2	58.5	881-1781 bp	1.0	82.1
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.2	58.5	879-1779 bp	1.0	82.1
psbE-petL_p5	CGAATAACCAACCCGCAATG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.8	58.5	880-1780 bp	1.0	82.0