Hotspot

petA-psbJ

LSC · 65948-67063 · 1116 bp

Back to Brocchinia

Pi 0.0165

MarkerSeek score 54.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 40
pi: 0.0165
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCAATCCCAATGTAGGAGGG	TCTGGGTTGGGTTCATCTCT	55.0	50.0	58.0	58.0	1301-1322 bp	1.0	83.9
petA-psbJ_p2	GCAATCCCAATGTAGGAGGG	TGGGTTGGGTTCATCTCTCT	55.0	50.0	58.0	58.0	1299-1320 bp	1.0	83.9
petA-psbJ_p3	CAGGGCCAGAACTTCTTGTT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.0	58.0	1361-1382 bp	1.0	83.7
petA-psbJ_p4	CAGGGCCAGAACTTCTTGTT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.0	58.0	1359-1380 bp	1.0	83.7
petA-psbJ_p5	GCAATCCCAATGTAGGAGGG	GGACTAGCTGTACCTACCGT	55.0	55.0	58.0	58.0	1730-1751 bp	1.0	83.0