Hotspot

trnS-GCU-trnG-UCC

LSC · 8762-10165 · 1404 bp

Back to Brocchinia

Pi 0.0229

MarkerSeek score 56.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1404
variable_sites: 61
pi: 0.0229
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GAGAAAGCGGAAAGAGAGGG	ACGAATCACACTTTTACCACT	55.0	38.1	58.0	55.5	813-1548 bp	1.0	59.2
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	758-1493 bp	1.0	59.2
trnS-GCU-trnG-UCC_p3	GAGAAAGCGGAAAGAGAGGG	AGAACGAATCACACTTTTACCAC	55.0	39.1	58.0	57.3	816-1551 bp	1.0	58.2
trnS-GCU-trnG-UCC_p4	GAGAAAGCGGAAAGAGAGGG	GAACGAATCACACTTTTACCACT	55.0	39.1	58.0	57.3	815-1550 bp	1.0	58.2
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	760-1495 bp	1.0	58.2