Hotspot

ndhF-rpl32

SSC · 104221-105120 · 900 bp

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Pi 0.0399
MarkerSeek score 69.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
900
variable_sites
35
pi
0.0399
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TTGTTGTTGCCCCGAAAAAC 55.0 45.0 58.1 58.0 1121-1124 bp 1.0 83.3
ndhF-rpl32_p2 GAAGAGGGAATTGTGCGGAT GTTTGTTGTTGCCCCGAAAA 50.0 45.0 58.0 58.0 1812-1815 bp 1.0 82.5
ndhF-rpl32_p3 GAAGAGGGAATTGTGCGGAT TTGTTGTTGCCCCGAAAAAC 50.0 45.0 58.0 58.0 1810-1813 bp 1.0 82.5
ndhF-rpl32_p4 AAAGCCCATATGCGACGAAT GTTTGTTGTTGCCCCGAAAA 45.0 45.0 58.0 58.0 1198 bp 0.5 59.6
ndhF-rpl32_p5 AAAGCCCATATGCGACGAAT TTGTTGTTGCCCCGAAAAAC 45.0 45.0 58.0 58.0 1196 bp 0.5 59.6