Hotspot

rpl22-rps19

IRb · 80200-80259 · 60 bp

Back to Briza

Pi 0.05
MarkerSeek score 70.10
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
60
variable_sites
3
pi
0.05
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TCCTTGCGAGTTTCCTTAGC TCACGAGTTATGAAAATGCAAGA 50.0 34.8 57.9 56.8 183-200 bp 1.0 47.0
rpl22-rps19_p2 AGTAAACGGTCGGTTGGAAC TCACGAGTTATGAAAATGCAAGA 50.0 34.8 58.1 56.8 205-222 bp 1.0 46.6
rpl22-rps19_p3 GTAAACGGTCGGTTGGAACT TCACGAGTTATGAAAATGCAAGA 50.0 34.8 58.1 56.8 204-221 bp 1.0 46.6
rpl22-rps19_p4 CCTTGCGAGTTTCCTTAGCA TCACGAGTTATGAAAATGCAAGA 50.0 34.8 58.2 56.8 182-199 bp 1.0 46.2
rpl22-rps19_p5 GCGAGTTTCCTTAGCACACT TCACGAGTTATGAAAATGCAAGA 50.0 34.8 58.2 56.8 178-195 bp 1.0 46.1