Hotspot

petG-trnW-CCA

LSC · 63627-63748 · 122 bp

Back to Briza

Pi 0.0902
MarkerSeek score 72.80
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
122
variable_sites
11
pi
0.0902
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 CGGGATTATTCGTGACTGCA AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.1 58.0 256 bp 1.0 83.4
petG-trnW-CCA_p2 CGGGATTATTCGTGACTGCA TATGGGCAGTAGCGAAGAGA 50.0 50.0 58.1 57.9 375 bp 1.0 82.4
petG-trnW-CCA_p3 TCCTGGTATTCTACGACGCT AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.0 58.0 443 bp 0.5 59.5
petG-trnW-CCA_p4 TCCTGGTATTCTACGACGCT TATGGGCAGTAGCGAAGAGA 50.0 50.0 58.0 57.9 562 bp 0.5 59.1
petG-trnW-CCA_p5 TCCTGGTATTCTACGACGCT TATCCCGCATCGAGAATCCT 50.0 50.0 58.0 58.1 1006 bp 0.5 59.0