Pi
0.0354
MarkerSeek score
66.40
Primer available
yes
Label
petA-psbJ
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| petA-psbJ_p1 | GCCTTTTGTTCTTCTTCGCA | GGATTGGGTTCATCTCTATAG | 45.0 | 42.9 | 57.2 | 52.6 | 919-925 bp | 1.0 | 43.0 |
| petA-psbJ_p2 | CGCATCTGTTATTTTGGCACA | GGATTGGGTTCATCTCTATAG | 42.9 | 42.9 | 57.8 | 52.6 | 903-909 bp | 1.0 | 43.0 |
| petA-psbJ_p3 | GCCTTTTGTTCTTCTTCGCAT | GGATTGGGTTCATCTCTATAG | 42.9 | 42.9 | 57.7 | 52.6 | 919-925 bp | 1.0 | 43.0 |
| petA-psbJ_p4 | GGCCTTTTGTTCTTCTTCGC | GGATTGGGTTCATCTCTATAG | 50.0 | 42.9 | 57.9 | 52.6 | 920-926 bp | 1.0 | 42.9 |
| petA-psbJ_p5 | TCCAAGGCCTTTTGTTCTTCT | GGATTGGGTTCATCTCTATAG | 42.9 | 42.9 | 57.7 | 52.6 | 925-931 bp | 1.0 | 42.9 |