Hotspot

ndhC-trnV-UAC

LSC · 49658-50567 · 910 bp

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Pi 0.0382
MarkerSeek score 65.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
910
variable_sites
26
pi
0.0382
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.75
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 36.4 55.0 56.1 57.9 733-955 bp 1.0 54.8
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA CGGTTCGAATCCGTATAGCC 36.4 55.0 56.1 58.3 734-956 bp 1.0 52.9
ndhC-trnV-UAC_p3 CATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 38.1 55.0 54.6 57.9 732-954 bp 1.0 50.8
ndhC-trnV-UAC_p4 ATCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 734-956 bp 1.0 50.7
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 733-955 bp 1.0 50.7