Hotspot

trnF-GAA-ndhJ

LSC · 47380-47965 · 586 bp

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Pi 0.0393
MarkerSeek score 70.70
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
586
variable_sites
23
pi
0.0393
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA ACGTAAGGACTATATAACCCCCA 50.0 43.5 58.1 57.9 672-680 bp 1.0 57.8
trnF-GAA-ndhJ_p2 AATCCTCGTGTCACCAGTTC ACGTAAGGACTATATAACCCCCA 50.0 43.5 57.5 57.9 675-683 bp 1.0 54.5
trnF-GAA-ndhJ_p3 TCAGTTGGTAGAGCAGAGGA ACGTAAGGACTATATAACCCCCA 50.0 43.5 57.4 57.9 700-708 bp 1.0 53.3
trnF-GAA-ndhJ_p4 TGGTAGAGCAGAGGACTGAA ACGTAAGGACTATATAACCCCCA 50.0 43.5 57.4 57.9 695-703 bp 1.0 53.3
trnF-GAA-ndhJ_p5 TTGGTAGAGCAGAGGACTGA ACGTAAGGACTATATAACCCCCA 50.0 43.5 57.4 57.9 696-704 bp 1.0 53.3