Hotspot

ADO75_gt016-trnF-GAA

LSC · 46962-47306 · 345 bp

Back to Briza

Pi 0.0437
MarkerSeek score 73.90
Primer available yes
Label ADO75_gt016-trnF-GAA

Feature Metadata

label_name
ADO75_gt016-trnF-GAA
feature_type
igs
region
LSC
length_bp
345
variable_sites
15
pi
0.0437
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ADO75_gt016-trnF-GAA_p1 AAGTCGTGAGGGTTCAAGTC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 434-436 bp 1.0 78.5
ADO75_gt016-trnF-GAA_p2 AAGTCGTGAGGGTTCAAGTC CTGCCAGGAACCAGATTTGA 50.0 50.0 57.5 57.8 449-451 bp 1.0 77.2
ADO75_gt016-trnF-GAA_p3 AAGTCGTGAGGGTTCAAGTC TCTGCCAGGAACCAGATTTG 50.0 50.0 57.5 57.8 450-452 bp 1.0 77.2
ADO75_gt016-trnF-GAA_p4 AAGTCGTGAGGGTTCAAGTC TTTTCTCTGCCAGGAACCAG 50.0 50.0 57.5 57.7 455-457 bp 1.0 76.5
ADO75_gt016-trnF-GAA_p5 AAGTCGTGAGGGTTCAAGTC TCTCTGCCAGGAACCAGATT 50.0 50.0 57.5 58.3 452-454 bp 1.0 76.1