Hotspot

trnT-UGU-ADO75_gt016

LSC · 45661-46458 · 798 bp

Back to Briza

Pi 0.031
MarkerSeek score 72.40
Primer available yes
Label trnT-UGU-ADO75_gt016

Feature Metadata

label_name
trnT-UGU-ADO75_gt016
feature_type
igs
region
LSC
length_bp
798
variable_sites
22
pi
0.031
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-ADO75_gt016_p1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.0 58.0 854-899 bp 1.0 85.8
trnT-UGU-ADO75_gt016_p2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.1 58.0 846-891 bp 1.0 85.4
trnT-UGU-ADO75_gt016_p3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 55.0 50.0 57.9 58.0 824-869 bp 1.0 85.0
trnT-UGU-ADO75_gt016_p4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 50.0 55.0 58.0 58.2 844-889 bp 1.0 83.9
trnT-UGU-ADO75_gt016_p5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 50.0 50.0 58.0 57.7 853-898 bp 1.0 83.6