Hotspot

psbI-trnS-GCU

LSC · 7540-7648 · 109 bp

Back to Briza

Pi 0.055
MarkerSeek score 73.90
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
109
variable_sites
6
pi
0.055
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 TCTAATGACCCAGGACGGAA TAAAGCGGCGGATTGCTAAT 50.0 45.0 57.8 58.0 216-228 bp 1.0 79.9
psbI-trnS-GCU_p2 ATCTAATGACCCAGGACGGA TAAAGCGGCGGATTGCTAAT 50.0 45.0 57.5 58.0 217-229 bp 1.0 78.1
psbI-trnS-GCU_p3 TCTAATGACCCAGGACGGAA ATGGCTGAGTGGACTAAAGC 50.0 50.0 57.8 57.6 230-242 bp 1.0 76.8
psbI-trnS-GCU_p4 TCTAATGACCCAGGACGGAA GAGAGATGGCTGAGTGGACT 50.0 55.0 57.8 58.5 235-247 bp 1.0 75.9
psbI-trnS-GCU_p5 ATCTAATGACCCAGGACGGA ATGGCTGAGTGGACTAAAGC 50.0 50.0 57.5 57.6 231-243 bp 1.0 75.0