Hotspot

ndhF-rpl32

SSC · 112355-113337 · 983 bp

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Pi 0.0114
MarkerSeek score 60.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
983
variable_sites
20
pi
0.0114
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGCGTAATTAAAGGTAAAATCCATGA GTACGTTTCTTTGGAACTGCC 30.8 47.6 57.5 57.7 1009-1053 bp 1.0 45.5
ndhF-rpl32_p2 AGCGTAATTAAAGGTAAAATCCA GTACGTTTCTTTGGAACTGCC 30.4 47.6 54.3 57.7 1009-1053 bp 1.0 45.5
ndhF-rpl32_p3 GCGTAATTAAAGGTAAAATCCATGA GTACGTTTCTTTGGAACTGCC 32.0 47.6 56.2 57.7 1008-1052 bp 1.0 45.5
ndhF-rpl32_p4 AGCGTAATTAAAGGTAAAATCCATG GTACGTTTCTTTGGAACTGCC 32.0 47.6 56.2 57.7 1009-1053 bp 1.0 45.5
ndhF-rpl32_p5 GAAGCGTAATTAAAGGTAAAATCCA GTACGTTTCTTTGGAACTGCC 32.0 47.6 56.2 57.7 1011-1055 bp 1.0 45.5