Hotspot

clpP-psbB

LSC · 72418-72901 · 484 bp

Back to Breynia

Pi 0.0251
MarkerSeek score 74.00
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
484
variable_sites
24
pi
0.0251
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGGACAAGTCGCACTATACG GACAGTAAACGACCGGGATC 55.0 55.0 58.5 58.1 631-722 bp 1.0 78.1
clpP-psbB_p2 CGGACAAGTCGCACTATACG AACCAGCAACCAAAGCAGTA 55.0 45.0 58.5 57.9 666-757 bp 1.0 78.0
clpP-psbB_p3 GGACAAGTCGCACTATACGT AACCAGCAACCAAAGCAGTA 50.0 45.0 57.4 57.9 665-756 bp 1.0 77.9
clpP-psbB_p4 GGACAAGTCGCACTATACGT GACAGTAAACGACCGGGATC 50.0 55.0 57.4 58.1 630-721 bp 1.0 77.9
clpP-psbB_p5 CGGACAAGTCGCACTATACG CGATACCAAGGCAAACCCAT 55.0 50.0 58.5 58.2 589-680 bp 1.0 77.0