Hotspot

rps12-clpP

LSC · 70221-70423 · 203 bp

Back to Breynia

Pi 0.0197
MarkerSeek score 58.00
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
203
variable_sites
7
pi
0.0197
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 TCTGATTGGCTGTCTTGTGT GATCTTGTAGCAGTTGCATA 45.0 40.0 57.1 53.1 267-290 bp 1.0 46.0
rps12-clpP_p2 TCTGATTGGCTGTCTTGTGT GATCTTGTAGCAGTTGCATAA 45.0 38.1 57.1 54.0 267-290 bp 1.0 46.0
rps12-clpP_p3 TCTGATTGGCTGTCTTGTGTT GATCTTGTAGCAGTTGCATA 42.9 40.0 57.8 53.1 267-290 bp 1.0 46.0
rps12-clpP_p4 TTCTGATTGGCTGTCTTGTGT GATCTTGTAGCAGTTGCATA 42.9 40.0 57.8 53.1 268-291 bp 1.0 46.0
rps12-clpP_p5 TCTGATTGGCTGTCTTGTGTT GATCTTGTAGCAGTTGCATAA 42.9 38.1 57.8 54.0 267-290 bp 1.0 46.0